Plasmid_Backbone

Part:BBa_K5308006:Design

Designed by: Chengyao Yin   Group: iGEM24_SZ-SHD   (2024-10-02)


HapAmp backbone for integration in yeast genome with high copynumber


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2650
    Illegal XbaI site found at 1874
    Illegal SpeI site found at 5602
    Illegal PstI site found at 1140
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2650
    Illegal NheI site found at 817
    Illegal SpeI site found at 5602
    Illegal PstI site found at 1140
    Illegal NotI site found at 2062
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2650
    Illegal BglII site found at 1709
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2650
    Illegal XbaI site found at 1874
    Illegal SpeI site found at 5602
    Illegal PstI site found at 1140
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2650
    Illegal XbaI site found at 1874
    Illegal SpeI site found at 5602
    Illegal PstI site found at 1140
    Illegal AgeI site found at 1045
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 1742
    Illegal BsaI site found at 3966
    Illegal BsaI.rc site found at 1497
    Illegal SapI.rc site found at 5048


Design Notes

No


Source

https://www.nature.com/articles/s41467-022-30529-8

References